Catalog #Product Description

ICP1836

The antibody is developed from rabbits using hydrocortisone 3-(O-carboxymethyl) oxime-KLH conjugate as immunogen and affinity purified with hydrocortisone-agarose. The antibody can be utilized for the detection and quantification of hydrocortisone.

Competitive inhibition ELISA for quantification of hydrocortisone

Immobilized antibody: Hydrocortisone Antibody (ICP1836), 1 µg/mL

Antigen: Hydrocortisone-3-CMO-Biotin(ICP1858), 1:20,000

Detection antibody: Biotin Antibody, HRP (ICP0612), 0.25 µg/mL

IC50 of free hydrocortisone: 15 ng/mL

                   Structure of Immunogen

Species

Rabbit

Formulation

250 µg/mL in PBS with 50% glycerol

Immunogen

Hydrocortisone 3-(O-carboxymethyl) oxime-KLH

Purification

Antigen-specific affinity chromatography

Applications

ELISA

Immuno-affinity Chromatography (IAC)

Storage & Stability

Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. The expiration date is one year from the date of shipping if stored properly.

Scientific Description

Hydrocortisone is a C-21 steroid hormone involved in the female menstrual cycle, pregnancy (supporting gestation) and embryogenesis of humans and other species. Hydrocortisone belongs to class of hormones called progestogens and is a major naturally occurring human progestogen.