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Catalog# Product Description |
ICP0190 Crosstide (PRAATF) is a substrate of the PKB (AKT) and SGK kinase families. Affinity purified rabbit polyclonal anti-pCrosstide antibody is developed with phosphorylated crosstide (PRAApTF) and absorbed with non-phosphorylated crosstide. The purified antibody was conjugated to peroxidase (HRP) via reductive amination. The antibody has been utilized for non-radioactive PKB and SGK kinase assays. |
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Species
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Rabbit
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Formulation
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250 µg/mL in PBS, 50% glycerol
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Immunogen
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Phospho-crosstide (PRAApTF)-KLH
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Purification
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The antibody was affinity purified with PRAApTF on agarose followed by affinity absorption with the non-phosphorylated crosstide.
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Conjugation
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Acetylation of the primary amine with the active ester of biotin (NHS-activated d-biotin).
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Specificity
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The antibody only recognizes the phosphorylated crosstide, the product of SGK and PKB in a non-radioactive kinase assay. There are no cross reactions with the non-phosphorylated crosstide.
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Applications
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ELISA (0.25 - 1.0 µg/mL); WB (0.25 - 1.0 µg/mL); Non-radioactive AKT and SGK kinase assay
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Scientific Description
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Crosstide is a synthetic peptide with the sequence (GRPRTSSFAEG). Kinases phosphorylate crosstide to form phospho-crosstide. [1-3]
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Storage & Stability
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Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is one year from date of shipping if properly stored.
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Product Specific References |
1. Nature. 1995. 378: 785-789. doi:10.1038/378785a0 |
