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首页 >> 产品 >> 标记抗体抗原 >> HRP标记 >> Phospho-AKT(Ser473) Antibody, HRP Conjugates
Phospho-AKT(Ser473) Antibody, HRP Conjugates
产品编号: ICP0455
  • 100.00µg
    ¥4500.00
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详细介绍

Phospho-AKT (Ser473) Antibody, HRP

Catalog#

Product Description

ICP0455

PKB alpha (AKT1) is a member of the PKB family, which is responsive to cell activation. The anti-PKB alpha antibodies were raised with a peptide based on the sequence of the human PKB C-terminal end and affinity purified with the same peptide on agarose. The affinity purified antibodies are useful for detection and isolation of PKB alpha from cell lysates.

A, B : Western blot analysis of FAB treated cell lysate with anti-pPKB (Ser473). ICP0453 and a monoclonal anti-pPKB (Ser473) from other supplier.
C: 5 ng/mL of FAB (anti-receptor) treatment with 10s                       D: Control - lymphocytes with no treatment
IHC of Human Breast Cancer
Species
Rabbit
Formulation
250 µg/mL in PBS, 50% glycerol
Immunogen
Human PKB C-terminal peptide that is based on human PKB-KLH conjugates
Purification
The antibody was affinity purified with PKB phospho-peptide on agarose and affinity adsorption with non-phospho-peptide of the same sequence.
Specificity
Recognizes phosphorylated PKB on serine 473. There are no cross reactions with the non-phosphorylated PKB.
Applications
ELISA (0.25 µg/mL); WB (1 µg/mL); IP (10 µg/2 mg crude protein)
Scientific Description
AKT is also known as protein kinase B (PKB). It is an important molecule in mammalian cellular signalling. It inhibits apoptotic processes and is involved in cellular survival pathways. It also induces protein synthesis pathways.
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage,  store product at -20°C.  Expiration date is six months from date of shipping if stored properly.
Product Specific References
1. J. Clin. Invest. 2003. 112: 197-208. doi:10.1172/JCI16885


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