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Methylated Lysine Antibody, Biotin
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Catalog# Product Description |
ICP0503 The affinity purified rabbit polyclonal anti-methylated lysine antibody is developed using a technique unique to ImmuneChem. The methylated lysine antibodies are affinity purified using N-methyl (epsilon amino group) lysine on agarose as the affinity matrix. The purified antibody was conjugated to d-biotin via acetylation. Direct labelling of the primary antibody will avoid the use of secondary antibodies therefore eliminating the interference of the secondary antibody conjugates. |
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Species
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Rabbit
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Formulation
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250 µg/mL in PBS, 50% glycerol
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Immunogen
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Methylated KLH conjugates
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Purification
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Affinity purified with methyl-lysine and di-methyl-lysine on agarose
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Conjugation
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Acetylation with NHS-biotin; Biotin/Ab molar ratio - 10:1
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Specificity
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The antibody recognizes anti-MeK (biotin conjugate) and recognizes proteins methylated on their lysine residues (mono and di-methyl lysine). It does not cross react with acetylated proteins.
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Applications
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ELISA (0.25 - 1.0 µg/mL); WB (0.25 - 1.0 µg/mL)
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Scientific Description
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MeK is a conserved post-translational modification which is an important biochemical process for many protein-protein interactions. It is found in many proteins, for example calmodulin, cytochrome B, chromosomal proteins, non-histones as well as neural storage body proteins. It has been suggested that methylation of lysine plays an important role in gene silencing. [1-3]
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Storage & Stability
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Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is one year from date of shipping if properly stored.
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Product Specific References
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1. J. Biol. Chem. 2003. 278: 18346-18352. doi.10.1074/jbc.M300890200 2. J. Biol. Chem. 2002. 277: 34655-34657. doi:10.1074/jbc.C200433200 3. J. Biol. Chem. 2002. 277: 11621-11624. doi:10.1074/jbc.C200045200
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