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Catalog# Product Description |
ICP9806 Affinity purified rabbit polyclonal phospho-serine antibodies are developed using a phospho-serine-KLH conjugate and phosvitin mixture as the immunogen. The phospho-serine specific antibodies were affinity purified with serine-phosphorylated peptide immobilized on agarose. This affinity purified antibody showed higher affinity than phospho-serine antibodies from another source. |
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Detection of a phosphorylated protein kinase with anti-phosphoserine (ICP9806):
Lane1-2: Kinase untreated
Lane 3-6: kinase treated with phosphatase PP1G
Western blot analysis of phosphorylated proteins with UV-treated mouse cell lysates.
A: Control
B-D: Treatment with increased UV dose
E: Phosphatase inhibitor treatment
High affinity anti-phospho-serine antibody recognizes phospho-proteins (Carbonic anhydrase, phosvitin and α-casein) but not non-phospho proteins (ovalbumin, BSA, β-lactoglobulin, trypsinogen)
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Species
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Rabbit | ||
| Formulation |
250 µg/mL in PBS, 50% glycerol, pH 7, 0.01% NaN3
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| Immunogen |
Phospho-serine KLH and phosvitin mixture
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| Purification |
Affinity purified with phospho-tyrosine on agarose
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| Specificity |
The antibody recognizes proteins and peptides phosphorylated on serine residues. There are no cross reactions with phospho-tyrosine.
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| Tested |
Human breast cancer tissue and mouse cell
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| Applications |
ELISA (1:250); WB (1:250); IP (10 µg/mg crude protein); IHC (1:50)
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| Scientific Description |
Serine is one of the 20 naturally occurring amino acids. Kinases phosphorylate serine to phospho-serine which is responsible for various signalling processes in eukaryotes.
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| Storage & Stability |
Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is one year from date of shipping if stored properly.
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Product Specific References |
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