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Catalog# Product Description |
ICP0485 Affinity purified rabbit polyclonal anti-phospho-MBP antibody is developed with synthetic phospho-peptide of myelin basic protein as the immunogen. The sequence of the antigen peptide corresponds to the phosphorylated site of MPB by MAPK. The antibody is affinity purified with PR(pT)PPPSQGKGRGL. This peptide sequence is identical for most of the isoforms of mammalian myelin basic proteins. |
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Species
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Rabbit
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Formulation
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1000 µg/mL in TBS, 50% glycerol
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Immunogen
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Phospho-peptide (PRpTPPPSQGKGRGL) of MPB conjugated to KLH
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Purification
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Affinity purified with synthetic PR(pT)PPPSQGKGRGL peptide on agarose and absorbed by peptide PRTPPPSQGKGRGL on agarose
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Specificity
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The antibody recognizes the 20 kDa myelin basic proteins phosphorylated by ERK½ and does not cross react with the non-phosphorylated form of myelin basic proteins. Tested species: mouse, swine, human and rabbits.
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Applications
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ELISA (0.25 - 1.0 µg/mL); WB (0.25 - 1.0 µg/mL); IP
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Storage & Stability
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Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is one year from date of shipping if properly stored.
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